Boosting the hydroxyfatty acid synthesis in Escherichia coli by expression of Bacillus megaterium glucose dehydrogenase

Boosting the hydroxyfatty acid synthesis in Escherichia coli by expression of Bacillus megaterium glucose dehydrogenase

This study devised an effective method for the direct production of hydroxyfatty acids (HFAs) from free fatty acids (FFAs) using Escherichia coli. By employing fatty acid hydroxylase P450BM3 and glucose dehydrogenase (GDH) to convert FFAs to HFAs, high-specificity production of C12 HFAs was achieved...

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Bibliographic Details
Main Authors: Xiangwei Wang, Xiang Xing, Qinglin Ma
Format: Article
Language:English
Published: Taylor & Francis Group 2016-09-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Hydroxyfatty acids
hydroxylase
glucose dehydrogenase
NADPH regeneration
fadD knocking out
Online Access:http://dx.doi.org/10.1080/13102818.2016.1196121

Internet

http://dx.doi.org/10.1080/13102818.2016.1196121

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