De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.

Bibliographic Details
Main Authors: Wei He, Liang Zhang, Oscar D. Villarreal, Rongjie Fu, Ella Bedford, Jingzhuang Dou, Anish Y. Patel, Mark T. Bedford, Xiaobing Shi, Taiping Chen, Blaine Bartholomew, Han Xu
Format: Article
Language:English
Published: Nature Publishing Group 2019-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-12489-8

Internet

https://doi.org/10.1038/s41467-019-12489-8

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