De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.

Bibliographic Details
Main Authors:	Wei He, Liang Zhang, Oscar D. Villarreal, Rongjie Fu, Ella Bedford, Jingzhuang Dou, Anish Y. Patel, Mark T. Bedford, Xiaobing Shi, Taiping Chen, Blaine Bartholomew, Han Xu
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-10-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-12489-8

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spelling	doaj-8f9bec4646d34e8fa3bf3716402d60502021-05-11T12:34:37ZengNature Publishing GroupNature Communications2041-17232019-10-0110111010.1038/s41467-019-12489-8De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screensWei He0Liang Zhang1Oscar D. Villarreal2Rongjie Fu3Ella Bedford4Jingzhuang Dou5Anish Y. Patel6Mark T. Bedford7Xiaobing Shi8Taiping Chen9Blaine Bartholomew10Han Xu11Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterCenter for Epigenetics, Van Andel Research InstituteDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterDepartment of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer CenterTiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.https://doi.org/10.1038/s41467-019-12489-8
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Wei He Liang Zhang Oscar D. Villarreal Rongjie Fu Ella Bedford Jingzhuang Dou Anish Y. Patel Mark T. Bedford Xiaobing Shi Taiping Chen Blaine Bartholomew Han Xu
spellingShingle	Wei He Liang Zhang Oscar D. Villarreal Rongjie Fu Ella Bedford Jingzhuang Dou Anish Y. Patel Mark T. Bedford Xiaobing Shi Taiping Chen Blaine Bartholomew Han Xu De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens Nature Communications
author_facet	Wei He Liang Zhang Oscar D. Villarreal Rongjie Fu Ella Bedford Jingzhuang Dou Anish Y. Patel Mark T. Bedford Xiaobing Shi Taiping Chen Blaine Bartholomew Han Xu
author_sort	Wei He
title	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_short	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_full	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_fullStr	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_full_unstemmed	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_sort	de novo identification of essential protein domains from crispr-cas9 tiling-sgrna knockout screens
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-10-01
description	Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.
url	https://doi.org/10.1038/s41467-019-12489-8
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De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

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