Optimization of Spheroid Colony Culture and Cryopreservation of Nucleus Pulposus Cells for the Development of Intervertebral Disc Regenerative Therapeutics

Optimization of Spheroid Colony Culture and Cryopreservation of Nucleus Pulposus Cells for the Development of Intervertebral Disc Regenerative Therapeutics

After the discovery of functionally superior Tie2-positive nucleus pulposus (NP) progenitor cells, new methods were needed to enable mass culture and cryopreservation to maintain these cells in an undifferentiated state with high cell yield. We used six types of EZSPHERE<sup>®</sup> dish...

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Bibliographic Details
Main Authors: Kosuke Sako, Daisuke Sakai, Yoshihiko Nakamura, Erika Matsushita, Jordy Schol, Takayuki Warita, Natsumi Horikita, Masato Sato, Masahiko Watanabe
Format: Article
Language:English
Published: MDPI AG 2021-04-01
Series:Applied Sciences
Subjects:
intervertebral disc
nucleus pulposus
nucleus pulposus progenitor cells
cryopreservation
Tie2
spheroid colony
Online Access:https://www.mdpi.com/2076-3417/11/8/3309

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https://www.mdpi.com/2076-3417/11/8/3309

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