Measurement of cholic acid synthesis and secretion by isolated rat hepatocytes.

Liver cells isolated from normal and cholestyramine-treated rats were incubated as cell suspensions for up to 4 hr in a simple, defined medium. The bile acid concentration in cells plus cell medium was determined by gas-liquid chromatography. Normal hepatocytes synthesized cholic acid at an initial...

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Bibliographic Details
Main Authors: M J Whiting, A M Edwards
Format: Article
Language:English
Published: Elsevier 1979-09-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520400227