Amplified insert assembly: an optimized approach to standard assembly of BioBrick<sup>TM</sup> genetic circuits
<p>Abstract</p> <p>A modified BioBrick™ assembly method was developed with higher fidelity than current protocols. The method utilizes a PCR reaction with a standard primer set to amplify the inserted part. Background colonies are reduced by a combination of dephosphorylation and d...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-12-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | http://www.jbioleng.org/content/5/1/17 |