Amplified insert assembly: an optimized approach to standard assembly of BioBrick<sup>TM</sup> genetic circuits
<p>Abstract</p> <p>A modified BioBrick™ assembly method was developed with higher fidelity than current protocols. The method utilizes a PCR reaction with a standard primer set to amplify the inserted part. Background colonies are reduced by a combination of dephosphorylation and d...
Main Authors: | Speer Michael A, Richard Tom L |
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Format: | Article |
Language: | English |
Published: |
BMC
2011-12-01
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Series: | Journal of Biological Engineering |
Subjects: | |
Online Access: | http://www.jbioleng.org/content/5/1/17 |
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