Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Abstract Background Loop mediated isothermal amplification of nucleic acid templates is a rapid, sensitive and specific method suitable for molecular diagnostics. However the complexity of primer design and the number of primers involved can lead to false positives from non-specific primer interacti...

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Bibliographic Details
Main Authors: Patrick Hardinge, James A. H. Murray
Format: Article
Language:English
Published: BMC 2019-08-01
Series:BMC Biotechnology
Subjects:
Loop-mediated amplification (LAMP)
Molecular beacons
DNA
Genetically modified (GM) crops
Fluorescence
Online Access:http://link.springer.com/article/10.1186/s12896-019-0549-z

Internet

http://link.springer.com/article/10.1186/s12896-019-0549-z

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