Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination

Here, the authors present a two-photon light-sheet microscopy with an extended Bessel beam for a tunable field of view and reduced photodamage. They demonstrate long-term imaging of cellular dynamics over the whole body of medaka fish.

Bibliographic Details
Main Authors: Sota Takanezawa, Takashi Saitou, Takeshi Imamura
Format: Article
Language:English
Published: Nature Publishing Group 2021-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-23249-y