Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters
Rat embryonic stem cells (ESCs) offer the potential for sophisticated genome engineering in this valuable biomedical model species. However, germline transmission has been rare following conventional homologous recombination and clonal selection. Here, we used the CRISPR/Cas9 system to target genomi...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2017-10-01
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Series: | Stem Cell Reports |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671117304125 |