Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters
Rat embryonic stem cells (ESCs) offer the potential for sophisticated genome engineering in this valuable biomedical model species. However, germline transmission has been rare following conventional homologous recombination and clonal selection. Here, we used the CRISPR/Cas9 system to target genomi...
Main Authors: | Yaoyao Chen, Sonia Spitzer, Sylvia Agathou, Ragnhildur Thora Karadottir, Austin Smith |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2017-10-01
|
Series: | Stem Cell Reports |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671117304125 |
Similar Items
-
Efficient chimeric mouse production using a novel embryonic stem cell line
by: G. V. Kontsevaya, et al.
Published: (2017-02-01) -
Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells
by: Tsutomu Motohashi, et al.
Published: (2016-03-01) -
CRISPR/Cas9-AAV Mediated Knock-in at NRL Locus in Human Embryonic Stem Cells
by: Xianglian Ge, et al.
Published: (2016-01-01) -
A Modified Monomeric Red Fluorescent Protein Reporter for Assessing CRISPR Activity
by: Camilla Højland Knudsen, et al.
Published: (2018-05-01) -
GENOME EDITING TECHNOLOGY IN PLANT BREEDING: A REVIEW
by: Regmi R., et al.
Published: (2021-05-01)