Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters

Gene Editing in Rat Embryonic Stem Cells to Produce In Vitro Models and In Vivo Reporters

Rat embryonic stem cells (ESCs) offer the potential for sophisticated genome engineering in this valuable biomedical model species. However, germline transmission has been rare following conventional homologous recombination and clonal selection. Here, we used the CRISPR/Cas9 system to target genomi...

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Bibliographic Details
Main Authors: Yaoyao Chen, Sonia Spitzer, Sylvia Agathou, Ragnhildur Thora Karadottir, Austin Smith
Format: Article
Language:English
Published: Elsevier 2017-10-01
Series:Stem Cell Reports
Subjects:
gene targeting
CRISPR/Cas9
oligodendrocyte
neural crest
rat
transgenesis
homologous recombination
pluripotency
SOX10
embryonic stem cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671117304125

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