Molecular detection of human papillomavirus (HPV) in highly fragmented DNA from cervical cancer biopsies using double-nested PCR

Archived Formalin-Fixed Paraffin-Embedded (FFPE) tissue specimens can be a valuable source of human papillomavirus (HPV) nucleic acids for molecular biological analyses in retrospective studies. Although successful amplification with polymerase chain reaction (PCR) is essential for analysis of HPV D...

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Bibliographic Details
Main Authors: Leabaneng Tawe, Surbhi Grover, Mohan Narasimhamurthy, Sikhulile Moyo, Simani Gaseitsiwe, Ishmael Kasvosve, Giacomo M. Paganotti
Format: Article
Language:English
Published: Elsevier 2018-01-01
Series:MethodsX
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016118300852