Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals

Measurement of cytokine gene expression by reverse transcription quantitative polymerase chain reaction (RT-qPCR) is used widely to assess the immune system of animals and to identify biomarkers of disease, but its application is limited in wildlife species due to a lack of species-specific reagents...

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Bibliographic Details
Main Authors: María-Ignacia Meza Cerda, Rachael Gray, Damien P. Higgins
Format: Article
Language:English
Published: PeerJ Inc. 2020-11-01
Series:PeerJ
Subjects:
Online Access:https://peerj.com/articles/10306.pdf