Monitoring hepatic cholesterol 7α-hydroxylase activity by assay of the stable bile acid intermediate 7α-hydroxy-4-cholesten-3-one in peripheral blood

We describe an accurate method for monitoring the enzymatic activity of hepatic cholesterol 7α-hydroxylase (C7αOH; CYP7A1), the rate-limiting and major regulatory enzyme in the synthesis of bile acids. Assay of 7α-hydroxy-4-cholesten-3-one (C4), an intermediate in bile acid synthesis, revealed that...

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Bibliographic Details
Main Authors: Cecilia Gälman, Ingela Arvidsson, Bo Angelin, Mats Rudling
Format: Article
Language:English
Published: Elsevier 2003-04-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520311779