A Redox-Neutral, Two-Enzyme Cascade for the Production of Malate and Gluconate from Pyruvate and Glucose
A triple mutant of NADP(H)-dependent malate dehydrogenase from thermotolerant <i>Thermococcus kodakarensis</i> has an altered cofactor preference for NAD<sup>+</sup>, as well as improved malate production compared to wildtype malate dehydrogenase. By combining mutant malate d...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
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| Series: | Applied Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3417/11/11/4877 |