Comprehensive Luciferase-Based Reporter Gene Assay Reveals Previously Masked Up-Regulatory Effects of miRNAs

MicroRNAs (miRNAs) are small non-coding RNAs that regulate the majority of the transcriptome at a post-transcriptional level. Because of this critical role, it is important to ensure that the assays used to determine their functionality are robust and reproducible. Typically, the reporter gene assay...

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Bibliographic Details
Main Authors: Danae Campos-Melo, Cristian A. Droppelmann, Kathryn Volkening, Michael J. Strong
Format: Article
Language:English
Published: MDPI AG 2014-09-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:http://www.mdpi.com/1422-0067/15/9/15592