Antibacterial susceptability of E. coli strains isolated from raw milk
Introduction. The processing of most raw milk products can lead to contamination with unwanted microflora due to poor sanitation and hygienic conditions. The inadequate antibiotic use over the past decades has led to the emergence and wide spread of bacterial populations, particularly of Escherichia...
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Asociatia de Biosiguranta si Biosecuritate
2021-06-01
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doaj-91895aacf521486384904a7e5e5bb7b12021-06-18T08:37:30ZengAsociatia de Biosiguranta si BiosecuritateOne Health & Risk Management2887-34582587-34662021-06-0123485410.38045/ohrm.2021.3.08Antibacterial susceptability of E. coli strains isolated from raw milkValerii USHKALOV0https://orcid.org/0000-0001-5694-632XVyacheslav DANCHUK1https://orcid.org/0000-0003-2156-1758Artem USHKALOV2https://orcid.org/0000-0001-8317-7909 Aidyn SALMANOV3https://orcid.org/0000-0002-4673-1154Yuriy VISHOVAN4https://orcid.org/0000-0003-1128-593XSergiy BOIANOVSKIY5https://orcid.org/0000-0002-4621-5192Sergiy TERESHCHENKO6https://orcid.org/0000-0002-5786-8711Liliana DAVYDOVSKA7https://orcid.org/0000-0003-5385-4500Ukrainian Laboratory of Quality and Safety of Agricultural Proucts, National University of Life and Environmental Sciences of UkraineUkrainian Laboratory of Quality and Safety of Agricultural Proucts, National University of Life and Environmental Sciences of UkraineState Service of Ukraine on Food Safety and Consumer Protection in Kharkiv region, UkraineDepartment of microbiology, epidemiology and infection control, Shupyk National Medical Academy of Postgraduate Education, Ukrainian National Committee of Infection Control and Antimicrobial Resistance, UkraineUkrainian Laboratory of Quality and Safety of Agricultural Proucts, National University of Life and Environmental Sciences of UkraineUkrainian Laboratory of Quality and Safety of Agricultural Proucts, National University of Life and Environmental Sciences of UkraineUkrainian Laboratory of Quality and Safety of Agricultural Proucts, National University of Life and Environmental Sciences of UkraineUkrainian Laboratory of Quality and Safety of Agricultural Proucts, National University of Life and Environmental Sciences of UkraineIntroduction. The processing of most raw milk products can lead to contamination with unwanted microflora due to poor sanitation and hygienic conditions. The inadequate antibiotic use over the past decades has led to the emergence and wide spread of bacterial populations, particularly of Escherichia coli, which developed resistance to antibacterial drugs. Material and methods. Raw milk samples were obtained from clinically healthy cows on farms from Kiev and Poltava regions to identify E . coli, Staphylococcus spp., Enterococcus spp. isolates. Antimicrobial susceptibility testing was performed using the EUCAST disk diffusion method and MU on “Determination of microbial susceptibility to antibacterial drugs”. Results. The examined milk samples revealed the presence of E . coli, Staphylococcus spp. and Enterococcus spp. isolates, which proves poor sanitary and hygienic conditions of milk production process. Escherichia coli isolates were found susceptible to Ampicillin/sulbactam, Cefoxitin (100%), Meropenem, Tobramycin (100%), Netilin, Tigecycline, Nitroxoline (100%), Gatifloxacin, and Nitrofurantoin (100%). The studied E. coli isolates were found resistant to Ampicillin (100%), Imipenem, Tetracycline, and Doxycycline (100%). 41.7% of isolates of Staphylococcus epidermidis, Staphylococcus aureus were found resistant to Oxacillin, of which 90% were resistant to Benzylpenicillin and 20% to Rifampicin. Conclusions. The circulation of antibiotic-resistant Enterobacteriaceae strains among farm animals is a major problem requiring a strategy development aimed to prevent the emergence and spread of antibiotic resistance worldwide.https://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/139/97escherichia colistrainsmicroorganismsraw milkantibiotic resistancesusceptibility |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Valerii USHKALOV Vyacheslav DANCHUK Artem USHKALOV Aidyn SALMANOV Yuriy VISHOVAN Sergiy BOIANOVSKIY Sergiy TERESHCHENKO Liliana DAVYDOVSKA |
spellingShingle |
Valerii USHKALOV Vyacheslav DANCHUK Artem USHKALOV Aidyn SALMANOV Yuriy VISHOVAN Sergiy BOIANOVSKIY Sergiy TERESHCHENKO Liliana DAVYDOVSKA Antibacterial susceptability of E. coli strains isolated from raw milk One Health & Risk Management escherichia coli strains microorganisms raw milk antibiotic resistance susceptibility |
author_facet |
Valerii USHKALOV Vyacheslav DANCHUK Artem USHKALOV Aidyn SALMANOV Yuriy VISHOVAN Sergiy BOIANOVSKIY Sergiy TERESHCHENKO Liliana DAVYDOVSKA |
author_sort |
Valerii USHKALOV |
title |
Antibacterial susceptability of E. coli strains isolated from raw milk |
title_short |
Antibacterial susceptability of E. coli strains isolated from raw milk |
title_full |
Antibacterial susceptability of E. coli strains isolated from raw milk |
title_fullStr |
Antibacterial susceptability of E. coli strains isolated from raw milk |
title_full_unstemmed |
Antibacterial susceptability of E. coli strains isolated from raw milk |
title_sort |
antibacterial susceptability of e. coli strains isolated from raw milk |
publisher |
Asociatia de Biosiguranta si Biosecuritate |
series |
One Health & Risk Management |
issn |
2887-3458 2587-3466 |
publishDate |
2021-06-01 |
description |
Introduction. The processing of most raw milk products can lead to contamination with unwanted microflora due to poor sanitation and hygienic conditions. The inadequate antibiotic use over the past decades has led to the emergence and wide spread of bacterial populations, particularly of Escherichia coli, which developed resistance to antibacterial drugs.
Material and methods. Raw milk samples were obtained from clinically healthy cows on farms from Kiev and Poltava regions to identify E . coli, Staphylococcus spp., Enterococcus spp. isolates. Antimicrobial susceptibility testing was performed using the EUCAST disk diffusion method and MU on “Determination of microbial susceptibility to antibacterial drugs”. Results. The examined milk samples revealed the presence of E . coli, Staphylococcus spp. and Enterococcus spp. isolates, which proves poor sanitary and hygienic conditions of milk production process. Escherichia coli isolates were found susceptible to Ampicillin/sulbactam, Cefoxitin (100%), Meropenem, Tobramycin (100%), Netilin, Tigecycline, Nitroxoline (100%), Gatifloxacin, and Nitrofurantoin (100%). The studied E. coli isolates were found resistant to Ampicillin (100%), Imipenem, Tetracycline, and Doxycycline (100%). 41.7% of isolates of Staphylococcus epidermidis, Staphylococcus aureus were found resistant to Oxacillin, of which 90% were resistant to Benzylpenicillin and 20% to Rifampicin. Conclusions.
The circulation of antibiotic-resistant Enterobacteriaceae strains among farm animals is a major problem requiring a strategy development aimed to prevent the emergence and spread of antibiotic resistance worldwide. |
topic |
escherichia coli strains microorganisms raw milk antibiotic resistance susceptibility |
url |
https://journal.ohrm.bba.md/index.php/journal-ohrm-bba-md/article/view/139/97 |
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