Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism
The many virtues that made the yeast Saccharomyces cerevisiae a dominant model organism for genetics and molecular biology, are now establishing its role in chemical genetics. Its experimental tractability (i.e., rapid doubling time, simple culture conditions) and the availability of powerful tools...
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2012-11-01
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Online Access: | http://www.mdpi.com/1420-3049/17/11/13098 |
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doaj-91a4be986223462e9662fb9654b1055b2020-11-24T23:42:42ZengMDPI AGMolecules1420-30492012-11-011711130981311510.3390/molecules171113098Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting MetabolismMarie EvangelistaCurt ScharfeUlrich SchlechtRobert St.OngeThe many virtues that made the yeast Saccharomyces cerevisiae a dominant model organism for genetics and molecular biology, are now establishing its role in chemical genetics. Its experimental tractability (i.e., rapid doubling time, simple culture conditions) and the availability of powerful tools for drug-target identification, make yeast an ideal organism for high-throughput phenotypic screening. It may be especially applicable for the discovery of chemical probes targeting highly conserved cellular processes, such as metabolism and bioenergetics, because these probes would likely inhibit the same processes in higher eukaryotes (including man). Importantly, changes in normal cellular metabolism are associated with a variety of diseased states (including neurological disorders and cancer), and exploiting these changes for therapeutic purposes has accordingly gained considerable attention. Here, we review progress and challenges associated with forward chemical genetic screening in yeast. We also discuss evidence supporting these screens as a useful strategy for discovery of new chemical probes and new druggable targets related to cellular metabolism.http://www.mdpi.com/1420-3049/17/11/13098yeastforward chemical geneticschemogenomic profilingglycolysiscancer metabolismWarburg effectmitochondriamethotrexateleucovorin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marie Evangelista Curt Scharfe Ulrich Schlecht Robert St.Onge |
spellingShingle |
Marie Evangelista Curt Scharfe Ulrich Schlecht Robert St.Onge Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism Molecules yeast forward chemical genetics chemogenomic profiling glycolysis cancer metabolism Warburg effect mitochondria methotrexate leucovorin |
author_facet |
Marie Evangelista Curt Scharfe Ulrich Schlecht Robert St.Onge |
author_sort |
Marie Evangelista |
title |
Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism |
title_short |
Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism |
title_full |
Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism |
title_fullStr |
Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism |
title_full_unstemmed |
Forward Chemical Genetics in Yeast for Discovery of Chemical Probes Targeting Metabolism |
title_sort |
forward chemical genetics in yeast for discovery of chemical probes targeting metabolism |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2012-11-01 |
description |
The many virtues that made the yeast Saccharomyces cerevisiae a dominant model organism for genetics and molecular biology, are now establishing its role in chemical genetics. Its experimental tractability (i.e., rapid doubling time, simple culture conditions) and the availability of powerful tools for drug-target identification, make yeast an ideal organism for high-throughput phenotypic screening. It may be especially applicable for the discovery of chemical probes targeting highly conserved cellular processes, such as metabolism and bioenergetics, because these probes would likely inhibit the same processes in higher eukaryotes (including man). Importantly, changes in normal cellular metabolism are associated with a variety of diseased states (including neurological disorders and cancer), and exploiting these changes for therapeutic purposes has accordingly gained considerable attention. Here, we review progress and challenges associated with forward chemical genetic screening in yeast. We also discuss evidence supporting these screens as a useful strategy for discovery of new chemical probes and new druggable targets related to cellular metabolism. |
topic |
yeast forward chemical genetics chemogenomic profiling glycolysis cancer metabolism Warburg effect mitochondria methotrexate leucovorin |
url |
http://www.mdpi.com/1420-3049/17/11/13098 |
work_keys_str_mv |
AT marieevangelista forwardchemicalgeneticsinyeastfordiscoveryofchemicalprobestargetingmetabolism AT curtscharfe forwardchemicalgeneticsinyeastfordiscoveryofchemicalprobestargetingmetabolism AT ulrichschlecht forwardchemicalgeneticsinyeastfordiscoveryofchemicalprobestargetingmetabolism AT robertstonge forwardchemicalgeneticsinyeastfordiscoveryofchemicalprobestargetingmetabolism |
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