Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants

Spelling Changes and Fluorescent Tagging With Prime Editing Vectors for Plants

Prime editing is an adaptation of the CRISPR-Cas system that uses a Cas9(H840A)-reverse transcriptase fusion and a guide RNA amended with template and primer binding site sequences to achieve RNA-templated conversion of the target DNA, allowing specified substitutions, insertions, and deletions. In...

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Bibliographic Details
Main Authors: Li Wang, Hilal Betul Kaya, Ning Zhang, Rhitu Rai, Matthew R. Willmann, Sara C. D. Carpenter, Andrew C. Read, Federico Martin, Zhangjun Fei, Jan E. Leach, Gregory B. Martin, Adam J. Bogdanove
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Genome Editing
Subjects:
prime editing
plant genome editing
fluorescent tagging
split GFP
Oryza sativa
Arabidopsis
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2021.617553/full

Internet

https://www.frontiersin.org/articles/10.3389/fgeed.2021.617553/full

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