Improved method for isolating high-quality RNA from mouse bone with RNAlater at room temperature

Accurate gene expression analysis of bone requires the ability to isolate RNA of good quality. Isolation of intact RNA from frozen bone tissue is problematic since RNA rapidly becomes degraded after thawing. Since we are interested in assessing gene expression from both bone marrow and mineralized b...

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Bibliographic Details
Main Authors: Kim B. Pedersen, Ashlee Williams, James Watt, Martin J. Ronis
Format: Article
Language:English
Published: Elsevier 2019-12-01
Series:Bone Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2352187219300178