Tropism of pandemic 2009 H1N1 influenza A virus
Substitutions at the receptor binding site of the pandemic H1N1 2009 influenza A virus (H1N1pdm) hemagglutinin (HA) gene may be critical in determining whether a virus binds to human or avian receptors. Previous reports suggest that HA Gly222 and/or Arg223 allow viruses to bind preferentially to the...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2012-04-01
|
Series: | Frontiers in Microbiology |
Subjects: | |
Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00128/full |
id |
doaj-91bca643b7a64caabd8ef546bcde267d |
---|---|
record_format |
Article |
spelling |
doaj-91bca643b7a64caabd8ef546bcde267d2020-11-24T21:24:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2012-04-01310.3389/fmicb.2012.0012822672Tropism of pandemic 2009 H1N1 influenza A virusRirin eRamadhany0Mayo eYasugi1Shota eNakamura2Shota eNakamura3Tomo eDaidoji4Tomo eDaidoji5Yohei eWatanabe6Kazuo eTakahashi7Kazuyoshi eIkuta8Takaaki eNakaya9Takaaki eNakaya10Takaaki eNakaya11Osaka UniversityOsaka UniversityOsaka UniversityOsaka UniversityOsaka UniversityKyoto prefectural University of MedicineOsaka UniversityOsaka Prefectural Institute of Public HealthOsaka UniversityOsaka UniversityKyoto prefectural University of MedicineOsaka UniversitySubstitutions at the receptor binding site of the pandemic H1N1 2009 influenza A virus (H1N1pdm) hemagglutinin (HA) gene may be critical in determining whether a virus binds to human or avian receptors. Previous reports suggest that HA Gly222 and/or Arg223 allow viruses to bind preferentially to the 2,3-linked sialic acid found in avian species. We also demonstrated that serial passaging of influenza A virus in embryonated chicken eggs increased viral growth 32-64-fold, coincident with the increased prevalence of Gly222 or Arg223 in HA protein. In this study, we showed that the minor genotype of 2,3 linkage-tropic viruses in upper airways became dominant after passaging through chicken eggs. Viruses possessing HA containing N125D-Q223R, N125D-D187E-Q223R, K119N-D222G and K119N-N129S-D222G, were detected in both clinical specimens and egg-passaged samples. These results might suggest that egg-adapted viruses, likely represented by 2,3 linkage-tropic virus, were also present in human upper airways as a minor population and transmitted in humans during the outbreak of H1N1pdm.http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00128/fullInfluenza VirusD222Ggeentic diversityH1N1pdmnext generation sequencer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ririn eRamadhany Mayo eYasugi Shota eNakamura Shota eNakamura Tomo eDaidoji Tomo eDaidoji Yohei eWatanabe Kazuo eTakahashi Kazuyoshi eIkuta Takaaki eNakaya Takaaki eNakaya Takaaki eNakaya |
spellingShingle |
Ririn eRamadhany Mayo eYasugi Shota eNakamura Shota eNakamura Tomo eDaidoji Tomo eDaidoji Yohei eWatanabe Kazuo eTakahashi Kazuyoshi eIkuta Takaaki eNakaya Takaaki eNakaya Takaaki eNakaya Tropism of pandemic 2009 H1N1 influenza A virus Frontiers in Microbiology Influenza Virus D222G geentic diversity H1N1pdm next generation sequencer |
author_facet |
Ririn eRamadhany Mayo eYasugi Shota eNakamura Shota eNakamura Tomo eDaidoji Tomo eDaidoji Yohei eWatanabe Kazuo eTakahashi Kazuyoshi eIkuta Takaaki eNakaya Takaaki eNakaya Takaaki eNakaya |
author_sort |
Ririn eRamadhany |
title |
Tropism of pandemic 2009 H1N1 influenza A virus |
title_short |
Tropism of pandemic 2009 H1N1 influenza A virus |
title_full |
Tropism of pandemic 2009 H1N1 influenza A virus |
title_fullStr |
Tropism of pandemic 2009 H1N1 influenza A virus |
title_full_unstemmed |
Tropism of pandemic 2009 H1N1 influenza A virus |
title_sort |
tropism of pandemic 2009 h1n1 influenza a virus |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2012-04-01 |
description |
Substitutions at the receptor binding site of the pandemic H1N1 2009 influenza A virus (H1N1pdm) hemagglutinin (HA) gene may be critical in determining whether a virus binds to human or avian receptors. Previous reports suggest that HA Gly222 and/or Arg223 allow viruses to bind preferentially to the 2,3-linked sialic acid found in avian species. We also demonstrated that serial passaging of influenza A virus in embryonated chicken eggs increased viral growth 32-64-fold, coincident with the increased prevalence of Gly222 or Arg223 in HA protein. In this study, we showed that the minor genotype of 2,3 linkage-tropic viruses in upper airways became dominant after passaging through chicken eggs. Viruses possessing HA containing N125D-Q223R, N125D-D187E-Q223R, K119N-D222G and K119N-N129S-D222G, were detected in both clinical specimens and egg-passaged samples. These results might suggest that egg-adapted viruses, likely represented by 2,3 linkage-tropic virus, were also present in human upper airways as a minor population and transmitted in humans during the outbreak of H1N1pdm. |
topic |
Influenza Virus D222G geentic diversity H1N1pdm next generation sequencer |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2012.00128/full |
work_keys_str_mv |
AT ririneramadhany tropismofpandemic2009h1n1influenzaavirus AT mayoeyasugi tropismofpandemic2009h1n1influenzaavirus AT shotaenakamura tropismofpandemic2009h1n1influenzaavirus AT shotaenakamura tropismofpandemic2009h1n1influenzaavirus AT tomoedaidoji tropismofpandemic2009h1n1influenzaavirus AT tomoedaidoji tropismofpandemic2009h1n1influenzaavirus AT yoheiewatanabe tropismofpandemic2009h1n1influenzaavirus AT kazuoetakahashi tropismofpandemic2009h1n1influenzaavirus AT kazuyoshieikuta tropismofpandemic2009h1n1influenzaavirus AT takaakienakaya tropismofpandemic2009h1n1influenzaavirus AT takaakienakaya tropismofpandemic2009h1n1influenzaavirus AT takaakienakaya tropismofpandemic2009h1n1influenzaavirus |
_version_ |
1725986270609408000 |