Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers

Multiplex Genetic Engineering Exploiting Pyrimidine Salvage Pathway-Based Endogenous Counterselectable Markers

This work reports the discovery of a novel genetic toolbox comprising multiple, endogenous selectable markers for targeted genomic insertions of DNAs of interest (DOIs). Marker genes encode proteins involved in 5-fluorocytosine uptake and pyrimidine salvage activities mediating 5-fluorocytosine deam...

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Bibliographic Details
Main Authors: Lukas Birštonas, Alex Dallemulle, Manuel S. López-Berges, Ilse D. Jacobsen, Martin Offterdinger, Beate Abt, Maria Straßburger, Ingo Bauer, Oliver Schmidt, Bettina Sarg, Herbert Lindner, Hubertus Haas, Fabio Gsaller
Format: Article
Language:English
Published: American Society for Microbiology 2020-04-01
Series:mBio
Subjects:
endogenous selectable markers
genetic engineering
pyrimidine salvage pathway
targeted genomic integrations
Online Access:https://doi.org/10.1128/mBio.00230-20

Internet

https://doi.org/10.1128/mBio.00230-20

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