Establishment of a tetracycline-off and heat shock-on gene expression system in tobacco
The tetracycline (Tet)-off gene expression regulation system based on the TetR-VP16/Top10 construct has not been widely utilized in plants, for its highly expressed TetR-VP16 activator is toxic to some plants and repeatedly replenishing tetracycline to turn off the constitutively active system is a...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2017-05-01
|
Series: | Journal of Integrative Agriculture |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311916615149 |