A Rapid Method for the Selection of Amidohydrolases from Metagenomic Libraries by Applying Synthetic Nucleosides and a Uridine Auxotrophic Host

A Rapid Method for the Selection of Amidohydrolases from Metagenomic Libraries by Applying Synthetic Nucleosides and a Uridine Auxotrophic Host

In this study, the development of a rapid, high-throughput method for the selection of amide-hydrolysing enzymes from the metagenome is described. This method is based on uridine auxotrophic <i>Escherichia coli</i> strain DH10B ∆<i>pyrFEC</i> and the use of <i>N</i&g...

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Bibliographic Details
Main Authors: Nina Urbelienė, Rita Meškienė, Matas Tiškus, Rūta Stanislauskienė, Agota Aučynaitė, Audrius Laurynėnas, Rolandas Meškys
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Catalysts
Subjects:
metagenomic libraries
amidohydrolase
amidase
selection method
high-throughput screening
Online Access:https://www.mdpi.com/2073-4344/10/4/445

Internet

https://www.mdpi.com/2073-4344/10/4/445

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