Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice
The anti-inflammatory effect of ginsenoside Rh2 (GRh2) has labeled it as one of the most important ginsenosides. The purpose of this study was to identify the anti-inflammatory and antioxidant effects of GRh2 using a lipopolysaccharide (LPS) challenge lung-injury animal model. GRh2 reduced LPS-induc...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-09-01
|
Series: | Nutrients |
Subjects: | |
Online Access: | http://www.mdpi.com/2072-6643/10/9/1208 |
id |
doaj-92ae02b78a6249a998dc2ceeb22727cb |
---|---|
record_format |
Article |
spelling |
doaj-92ae02b78a6249a998dc2ceeb22727cb2020-11-25T02:17:26ZengMDPI AGNutrients2072-66432018-09-01109120810.3390/nu10091208nu10091208Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in MiceYung-Hung Hsieh0Jeng-Shyan Deng1Yuan-Shiun Chang2Guan-Jhong Huang3Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 413, TaiwanDepartment of Food Nutrition and Health Biotechnology, Asia University, Taichung 413, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 413, TaiwanDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 413, TaiwanThe anti-inflammatory effect of ginsenoside Rh2 (GRh2) has labeled it as one of the most important ginsenosides. The purpose of this study was to identify the anti-inflammatory and antioxidant effects of GRh2 using a lipopolysaccharide (LPS) challenge lung-injury animal model. GRh2 reduced LPS-induced proinflammatory mediator nitric oxide (NO), tumor necrosis factor-alpha, interleukin (IL)-1β, and anti-inflammatory cytokines (IL-4, IL-6, and IL-10) production in lung tissues. GRh2 treatment decreased the histological alterations in the lung tissues and bronchoalveolar lavage fluid (BALF) protein content; total cell number also reduced in LPS-induced lung injury in mice. Moreover, GRh2 blocked iNOS, COX-2, the phosphorylation of IκB-α, ERK, JNK, p38, Raf-1, and MEK protein expression, which corresponds with the growth of HO-1, Nrf-2, catalase, SOD, and GPx expression in LPS-induced lung injury. An in vivo experimental study suggested that GRh2 has anti-inflammatory effects, and has potential therapeutic efficacy in major anterior segment lung diseases.http://www.mdpi.com/2072-6643/10/9/1208ginsenoside Rh2lipopolysaccharideacute lung injuryMEKNrf-2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yung-Hung Hsieh Jeng-Shyan Deng Yuan-Shiun Chang Guan-Jhong Huang |
spellingShingle |
Yung-Hung Hsieh Jeng-Shyan Deng Yuan-Shiun Chang Guan-Jhong Huang Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice Nutrients ginsenoside Rh2 lipopolysaccharide acute lung injury MEK Nrf-2 |
author_facet |
Yung-Hung Hsieh Jeng-Shyan Deng Yuan-Shiun Chang Guan-Jhong Huang |
author_sort |
Yung-Hung Hsieh |
title |
Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice |
title_short |
Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice |
title_full |
Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice |
title_fullStr |
Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice |
title_full_unstemmed |
Ginsenoside Rh2 Ameliorates Lipopolysaccharide-Induced Acute Lung Injury by Regulating the TLR4/PI3K/Akt/mTOR, Raf-1/MEK/ERK, and Keap1/Nrf2/HO-1 Signaling Pathways in Mice |
title_sort |
ginsenoside rh2 ameliorates lipopolysaccharide-induced acute lung injury by regulating the tlr4/pi3k/akt/mtor, raf-1/mek/erk, and keap1/nrf2/ho-1 signaling pathways in mice |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2018-09-01 |
description |
The anti-inflammatory effect of ginsenoside Rh2 (GRh2) has labeled it as one of the most important ginsenosides. The purpose of this study was to identify the anti-inflammatory and antioxidant effects of GRh2 using a lipopolysaccharide (LPS) challenge lung-injury animal model. GRh2 reduced LPS-induced proinflammatory mediator nitric oxide (NO), tumor necrosis factor-alpha, interleukin (IL)-1β, and anti-inflammatory cytokines (IL-4, IL-6, and IL-10) production in lung tissues. GRh2 treatment decreased the histological alterations in the lung tissues and bronchoalveolar lavage fluid (BALF) protein content; total cell number also reduced in LPS-induced lung injury in mice. Moreover, GRh2 blocked iNOS, COX-2, the phosphorylation of IκB-α, ERK, JNK, p38, Raf-1, and MEK protein expression, which corresponds with the growth of HO-1, Nrf-2, catalase, SOD, and GPx expression in LPS-induced lung injury. An in vivo experimental study suggested that GRh2 has anti-inflammatory effects, and has potential therapeutic efficacy in major anterior segment lung diseases. |
topic |
ginsenoside Rh2 lipopolysaccharide acute lung injury MEK Nrf-2 |
url |
http://www.mdpi.com/2072-6643/10/9/1208 |
work_keys_str_mv |
AT yunghunghsieh ginsenosiderh2ameliorateslipopolysaccharideinducedacutelunginjurybyregulatingthetlr4pi3kaktmtorraf1mekerkandkeap1nrf2ho1signalingpathwaysinmice AT jengshyandeng ginsenosiderh2ameliorateslipopolysaccharideinducedacutelunginjurybyregulatingthetlr4pi3kaktmtorraf1mekerkandkeap1nrf2ho1signalingpathwaysinmice AT yuanshiunchang ginsenosiderh2ameliorateslipopolysaccharideinducedacutelunginjurybyregulatingthetlr4pi3kaktmtorraf1mekerkandkeap1nrf2ho1signalingpathwaysinmice AT guanjhonghuang ginsenosiderh2ameliorateslipopolysaccharideinducedacutelunginjurybyregulatingthetlr4pi3kaktmtorraf1mekerkandkeap1nrf2ho1signalingpathwaysinmice |
_version_ |
1724886391043653632 |