SP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS

SP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS

Quantitative protein extraction from biological samples, as well as contaminants removal before LC-MS/MS, is fundamental for the successful bottom-up proteomic analysis. Four sample preparation methods, including the filter-aided sample preparation (FASP), two single-pot solid-phase-enhanced sample...

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Bibliographic Details
Main Authors: Kamil Mikulášek, Hana Konečná, David Potěšil, Renata Holánková, Jan Havliš, Zbyněk Zdráhal
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Plant Science
Subjects:
bottom-up
protein cleanup
Arabidopsis thaliana
sodium dodecyl sulfate removal
single-pot solid-phase-enhanced sample preparation
carboxylated magnetic beads
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.635550/full

Internet

https://www.frontiersin.org/articles/10.3389/fpls.2021.635550/full

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