Expression of the human molecular chaperone domain Bri2 BRICHOS on a gram per liter scale with an E. coli fed-batch culture

Expression of the human molecular chaperone domain Bri2 BRICHOS on a gram per liter scale with an E. coli fed-batch culture

Abstract Background The human Bri2 BRICHOS domain inhibits amyloid formation and toxicity and could be used as a therapeutic agent against amyloid diseases. For translation into clinical use, large quantities of correctly folded recombinant human (rh) Bri2 BRICHOS are required. To increase the expre...

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Bibliographic Details
Main Authors: Benjamin Schmuck, Gefei Chen, Josef Pelcman, Nina Kronqvist, Anna Rising, Jan Johansson
Format: Article
Language:English
Published: BMC 2021-07-01
Series:Microbial Cell Factories
Subjects:
Solubility tag
Bioreactor
High cell density culture
Protein expression
Protein purification
Online Access:https://doi.org/10.1186/s12934-021-01638-8

Internet

https://doi.org/10.1186/s12934-021-01638-8

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