Generation and isolation of single cells from mouse brain with mosaic analysis with double markers-induced uniparental chromosome disomy
Summary: Mosaic analysis with double markers (MADM) technology enables concomitant fluorescent cell labeling and induction of uniparental chromosome disomy (UPD) with single-cell resolution. In UPD, imprinted genes are either overexpressed 2-fold or are not expressed. Here, the MADM platform is util...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2020-12-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720302021 |