Saturated excitation microscopy using differential excitation for efficient detection of nonlinear fluorescence signals

We report a method to increase the efficiency of detecting nonlinear fluorescence signals in saturated excitation (SAX) microscopy. With this method, we compare fluorescence signals obtained under different degrees of saturated excitation to extract the nonlinear fluorescent signal induced by satura...

Full description

Bibliographic Details
Main Authors: Yasunori Nawa, Yasuo Yonemaru, Atsushi Kasai, Ryosuke Oketani, Hitoshi Hashimoto, Nicholas I. Smith, Katsumasa Fujita
Format: Article
Language:English
Published: AIP Publishing LLC 2018-08-01
Series:APL Photonics
Online Access:http://dx.doi.org/10.1063/1.5039567