Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitis
The greatest obstacle to an HIV cure is the persistence of latently infected cellular reservoirs in people living with HIV (PLWH) taking antiretroviral therapy (ART). However, no consensus exists on the direct link between local tissue inflammation and the HIV burden. Herein, we have compared the le...
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doaj-93a692a7477f4b6a97b5473dbc33881d2021-05-05T04:07:33ZengElsevierJournal of Virus Eradication2055-66402021-03-0171100033Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitisXiaorong Peng0Stéphane Isnard1John Lin2Brandon Fombuena3Talat Bessissow4Nicolas Chomont5Jean-Pierre Routy6Infectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada; Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, Canada; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang, ChinaInfectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada; Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, Canada; CIHR Canadian HIV Trials Network, Vancouver, BC, CanadaInfectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada; Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, CanadaInfectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada; Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, CanadaDivision of Gastroenterology, McGill University Health Center, Montréal, QC, CanadaCentre de Recherche du Centre Hospitalier de l’Université de Montréal, Montréal, QC, CanadaInfectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada; Chronic Viral Illness Service, McGill University Health Centre, Montréal, QC, Canada; Division of Hematology, McGill University Health Centre, Montréal, QC, Canada; Corresponding author. Infectious Diseases and Immunity in Global Health Program, Research Institute, McGill University Health Centre, Montréal, QC, Canada.The greatest obstacle to an HIV cure is the persistence of latently infected cellular reservoirs in people living with HIV (PLWH) taking antiretroviral therapy (ART). However, no consensus exists on the direct link between local tissue inflammation and the HIV burden. Herein, we have compared the levels of local inflammation, epithelial integrity and HIV DNA between inflamed and non-inflamed colon tissue in a PLWH who underwent a colectomy due to ulcerative colitis. We have observed a 27-fold higher frequency of cells harboring HIV DNA in inflamed compared to non-inflamed colon tissue. Analysis of the expression of occludin-1 and claudin-3 confirmed our macroscopic characterization of inflamed and non-inflamed colon. Our results confirm that increased gut permeability and inflammation are associated with a higher frequency of infected cells and suggest that restoring gut barrier integrity may be used as a strategy to reduce inflammation and HIV persistence in the gut.http://www.sciencedirect.com/science/article/pii/S2055664021000066HIV DNAReservoirColonUlcerative colitisInflammationGut barrier integrity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaorong Peng Stéphane Isnard John Lin Brandon Fombuena Talat Bessissow Nicolas Chomont Jean-Pierre Routy |
spellingShingle |
Xiaorong Peng Stéphane Isnard John Lin Brandon Fombuena Talat Bessissow Nicolas Chomont Jean-Pierre Routy Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitis Journal of Virus Eradication HIV DNA Reservoir Colon Ulcerative colitis Inflammation Gut barrier integrity |
author_facet |
Xiaorong Peng Stéphane Isnard John Lin Brandon Fombuena Talat Bessissow Nicolas Chomont Jean-Pierre Routy |
author_sort |
Xiaorong Peng |
title |
Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitis |
title_short |
Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitis |
title_full |
Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitis |
title_fullStr |
Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitis |
title_full_unstemmed |
Differences in HIV burden in the inflamed and non-inflamed colon from a person living with HIV and ulcerative colitis |
title_sort |
differences in hiv burden in the inflamed and non-inflamed colon from a person living with hiv and ulcerative colitis |
publisher |
Elsevier |
series |
Journal of Virus Eradication |
issn |
2055-6640 |
publishDate |
2021-03-01 |
description |
The greatest obstacle to an HIV cure is the persistence of latently infected cellular reservoirs in people living with HIV (PLWH) taking antiretroviral therapy (ART). However, no consensus exists on the direct link between local tissue inflammation and the HIV burden. Herein, we have compared the levels of local inflammation, epithelial integrity and HIV DNA between inflamed and non-inflamed colon tissue in a PLWH who underwent a colectomy due to ulcerative colitis. We have observed a 27-fold higher frequency of cells harboring HIV DNA in inflamed compared to non-inflamed colon tissue. Analysis of the expression of occludin-1 and claudin-3 confirmed our macroscopic characterization of inflamed and non-inflamed colon. Our results confirm that increased gut permeability and inflammation are associated with a higher frequency of infected cells and suggest that restoring gut barrier integrity may be used as a strategy to reduce inflammation and HIV persistence in the gut. |
topic |
HIV DNA Reservoir Colon Ulcerative colitis Inflammation Gut barrier integrity |
url |
http://www.sciencedirect.com/science/article/pii/S2055664021000066 |
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