Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants
Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of...
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doaj-93b3bc2abb0a409aa780c8576f92ac0c2021-04-30T08:31:47ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-04-011210.3389/fphar.2021.653064653064Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana PlantsNguyen-Quang-Duc Tien0Nguyen-Quang-Duc Tien1Moon-Sik Yang2Moon-Sik Yang3Yong-Suk Jang4Tae-Ho Kwon5Rajko Reljic6Mi-young Kim7Mi-young Kim8Department of Molecular Biology, Jeonbuk National University, Jeonju-Si, South KoreaUniversity of Sciences, Hue University, Hue City, Viet NamDepartment of Molecular Biology, Jeonbuk National University, Jeonju-Si, South KoreaNBM Inc., Wanjusandan 5-ro, Bongdong-eup, Wanju-Gun, Jeollabuk-do, South KoreaDepartment of Molecular Biology, Jeonbuk National University, Jeonju-Si, South KoreaNBM Inc., Wanjusandan 5-ro, Bongdong-eup, Wanju-Gun, Jeollabuk-do, South KoreaInstitute for Infection and Immunity, St George’s University of London, London, UKDepartment of Molecular Biology, Jeonbuk National University, Jeonju-Si, South KoreaInstitute for Infection and Immunity, St George’s University of London, London, UKPorcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered Nicotiana benthamiana plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) in vitro. The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.https://www.frontiersin.org/articles/10.3389/fphar.2021.653064/fullvaccinePEDVmucosalimmunityplant |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nguyen-Quang-Duc Tien Nguyen-Quang-Duc Tien Moon-Sik Yang Moon-Sik Yang Yong-Suk Jang Tae-Ho Kwon Rajko Reljic Mi-young Kim Mi-young Kim |
spellingShingle |
Nguyen-Quang-Duc Tien Nguyen-Quang-Duc Tien Moon-Sik Yang Moon-Sik Yang Yong-Suk Jang Tae-Ho Kwon Rajko Reljic Mi-young Kim Mi-young Kim Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants Frontiers in Pharmacology vaccine PEDV mucosal immunity plant |
author_facet |
Nguyen-Quang-Duc Tien Nguyen-Quang-Duc Tien Moon-Sik Yang Moon-Sik Yang Yong-Suk Jang Tae-Ho Kwon Rajko Reljic Mi-young Kim Mi-young Kim |
author_sort |
Nguyen-Quang-Duc Tien |
title |
Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants |
title_short |
Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants |
title_full |
Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants |
title_fullStr |
Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants |
title_full_unstemmed |
Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants |
title_sort |
systemic and oral immunogenicity of porcine epidemic diarrhea virus antigen fused to poly-fc of immunoglobulin g and expressed in δxt/ft nicotiana benthamiana plants |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Pharmacology |
issn |
1663-9812 |
publishDate |
2021-04-01 |
description |
Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered Nicotiana benthamiana plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) in vitro. The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection. |
topic |
vaccine PEDV mucosal immunity plant |
url |
https://www.frontiersin.org/articles/10.3389/fphar.2021.653064/full |
work_keys_str_mv |
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