Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants

Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants

Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of...

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Main Authors: Nguyen-Quang-Duc Tien, Moon-Sik Yang, Yong-Suk Jang, Tae-Ho Kwon, Rajko Reljic, Mi-young Kim
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-04-01
Series:Frontiers in Pharmacology
Subjects:
vaccine
PEDV
mucosal
immunity
plant
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2021.653064/full
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spelling doaj-93b3bc2abb0a409aa780c8576f92ac0c2021-04-30T08:31:47ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-04-011210.3389/fphar.2021.653064653064Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana PlantsNguyen-Quang-Duc Tien0Nguyen-Quang-Duc Tien1Moon-Sik Yang2Moon-Sik Yang3Yong-Suk Jang4Tae-Ho Kwon5Rajko Reljic6Mi-young Kim7Mi-young Kim8Department of Molecular Biology, Jeonbuk National University, Jeonju-Si, South KoreaUniversity of Sciences, Hue University, Hue City, Viet NamDepartment of Molecular Biology, Jeonbuk National University, Jeonju-Si, South KoreaNBM Inc., Wanjusandan 5-ro, Bongdong-eup, Wanju-Gun, Jeollabuk-do, South KoreaDepartment of Molecular Biology, Jeonbuk National University, Jeonju-Si, South KoreaNBM Inc., Wanjusandan 5-ro, Bongdong-eup, Wanju-Gun, Jeollabuk-do, South KoreaInstitute for Infection and Immunity, St George’s University of London, London, UKDepartment of Molecular Biology, Jeonbuk National University, Jeonju-Si, South KoreaInstitute for Infection and Immunity, St George’s University of London, London, UKPorcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered Nicotiana benthamiana plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) in vitro. The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.https://www.frontiersin.org/articles/10.3389/fphar.2021.653064/fullvaccinePEDVmucosalimmunityplant
collection DOAJ
language English
format Article
sources DOAJ
author Nguyen-Quang-Duc Tien
Nguyen-Quang-Duc Tien
Moon-Sik Yang
Moon-Sik Yang
Yong-Suk Jang
Tae-Ho Kwon
Rajko Reljic
Mi-young Kim
Mi-young Kim
spellingShingle Nguyen-Quang-Duc Tien
Nguyen-Quang-Duc Tien
Moon-Sik Yang
Moon-Sik Yang
Yong-Suk Jang
Tae-Ho Kwon
Rajko Reljic
Mi-young Kim
Mi-young Kim
Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants
Frontiers in Pharmacology
vaccine
PEDV
mucosal
immunity
plant
author_facet Nguyen-Quang-Duc Tien
Nguyen-Quang-Duc Tien
Moon-Sik Yang
Moon-Sik Yang
Yong-Suk Jang
Tae-Ho Kwon
Rajko Reljic
Mi-young Kim
Mi-young Kim
author_sort Nguyen-Quang-Duc Tien
title Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants
title_short Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants
title_full Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants
title_fullStr Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants
title_full_unstemmed Systemic and Oral Immunogenicity of Porcine Epidemic Diarrhea Virus Antigen Fused to Poly-Fc of Immunoglobulin G and Expressed in ΔXT/FT Nicotiana benthamiana Plants
title_sort systemic and oral immunogenicity of porcine epidemic diarrhea virus antigen fused to poly-fc of immunoglobulin g and expressed in δxt/ft nicotiana benthamiana plants
publisher Frontiers Media S.A.
series Frontiers in Pharmacology
issn 1663-9812
publishDate 2021-04-01
description Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered Nicotiana benthamiana plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) in vitro. The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.
topic vaccine
PEDV
mucosal
immunity
plant
url https://www.frontiersin.org/articles/10.3389/fphar.2021.653064/full
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