High yield expression and purification of recombinant human apolipoprotein A-II in Escherichia coli

High yield expression and purification of recombinant human apolipoprotein A-II in Escherichia coli

Recombinant expression systems have become powerful tools for understanding the structure and function of proteins, including the apolipoproteins that comprise human HDL. However, human apolipoprotein (apo)A-II has proven difficult to produce by recombinant techniques, likely contributing to our lac...

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Bibliographic Details
Main Authors: Loren E. Smith, Jun Yang, Leah Goodman, Xinqi Huang, Rong Huang, James Dressman, Jamie Morris, R. A. Gangani D. Silva, W. Sean Davidson, Giorgio Cavigiolio
Format: Article
Language:English
Published: Elsevier 2012-08-01
Series:Journal of Lipid Research
Subjects:
intein
fusion protein
affinity tag
bacteria
inexpensive
low temperature cleavage
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520418759

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http://www.sciencedirect.com/science/article/pii/S0022227520418759

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