Gas–liquid chromatographic assay of lipid-bound sialic acids: measurement of gangliosides in brain of several species

A method is described for analysis of gangliosides by GLC assay of the sialic acid component. Mild acid treatment in methanol converted the latter to methyl ketoside methyl ester, which was then chromatographed as the TMS derivative. The major methanolysis product was shown to be the β-anomer, and i...

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Bibliographic Details
Main Authors: ROBERT K. YU, ROBERT W. LEDEEN
Format: Article
Language:English
Published: Elsevier 1970-11-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520429335