Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban triatominae Optimización de la técnica de ADN polimórfico amplificado al azar (RAPD) para su utilización en la caracterización genética de triatomíneos cubanos

Random amplified polymorphic DNA (RAPD) technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of pr...

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Bibliographic Details
Main Authors: Jorge Fraga, Jinnay Rodriguez, Omar Fuentes, Aymé Fernandez-Calienes, Mayda Castex
Format: Article
Language:English
Published: Universidade de São Paulo 2005-10-01
Series:Revista do Instituto de Medicina Tropical de São Paulo
Subjects:
DNA
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652005000500010