Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban triatominae Optimización de la técnica de ADN polimórfico amplificado al azar (RAPD) para su utilización en la caracterización genética de triatomíneos cubanos
Random amplified polymorphic DNA (RAPD) technique is a simple and reliable method to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of assay. In this study, we analyzed the effect of changing the concentration of pr...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Universidade de São Paulo
2005-10-01
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Series: | Revista do Instituto de Medicina Tropical de São Paulo |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652005000500010 |