Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization
Summary: Generating robust CD4+ T-helper cell type 1 (Th1) responses is essential for protective vaccine-induced type 1 immunity. Here, we examine whether immunization formulation associated with enhanced vaccine efficacy promotes antigen targeting and cell recruitment into lymph node (LN) niches as...
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doaj-9420a957baf34ddb9e5d4ea8ab2984662020-11-25T03:17:49ZengElsevierCell Reports2211-12472020-05-01318Targeting Lymph Node Niches Enhances Type 1 Immune Responses to ImmunizationJeffrey Lian0Aleksandra J. Ozga1Caroline L. Sokol2Andrew D. Luster3Center for Immunology & Inflammatory Diseases, Division of Rheumatology, Allergy & Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA; Graduate Program in Immunology, Harvard Medical School, Boston, MA 02115, USACenter for Immunology & Inflammatory Diseases, Division of Rheumatology, Allergy & Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USACenter for Immunology & Inflammatory Diseases, Division of Rheumatology, Allergy & Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USACenter for Immunology & Inflammatory Diseases, Division of Rheumatology, Allergy & Immunology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA; Graduate Program in Immunology, Harvard Medical School, Boston, MA 02115, USA; Corresponding authorSummary: Generating robust CD4+ T-helper cell type 1 (Th1) responses is essential for protective vaccine-induced type 1 immunity. Here, we examine whether immunization formulation associated with enhanced vaccine efficacy promotes antigen targeting and cell recruitment into lymph node (LN) niches associated with optimal type 1 responses. Immunization with antigen and Toll-like receptor agonist emulsified in oil leads to an increased differentiation of IFNγ/TNF-α+ polyfunctional Th1 cells compared to an identical immunization in saline. Oil immunization results in a rapid delivery and persistence of antigen in interfollicular regions (IFRs) of the LN, whereas without oil, antigen is distributed in the medullary region. Following oil immunization, CXCL10-producing inflammatory monocytes accumulate in the IFR, which mobilizes antigen-specific CD4+ T cells into this niche. In this microenvironment, CD4+ T cells are advantageously positioned to encounter arriving IL-12-producing inflammatory dendritic cells (DCs). These data suggest that formulations delivering antigen to the LN IFR create an inflammatory niche that can improve vaccine efficacy.http://www.sciencedirect.com/science/article/pii/S221112472030632Xvaccinesemulsificationlymph nodeinterfollicular regionchemokinesCXCL10 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeffrey Lian Aleksandra J. Ozga Caroline L. Sokol Andrew D. Luster |
spellingShingle |
Jeffrey Lian Aleksandra J. Ozga Caroline L. Sokol Andrew D. Luster Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization Cell Reports vaccines emulsification lymph node interfollicular region chemokines CXCL10 |
author_facet |
Jeffrey Lian Aleksandra J. Ozga Caroline L. Sokol Andrew D. Luster |
author_sort |
Jeffrey Lian |
title |
Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization |
title_short |
Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization |
title_full |
Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization |
title_fullStr |
Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization |
title_full_unstemmed |
Targeting Lymph Node Niches Enhances Type 1 Immune Responses to Immunization |
title_sort |
targeting lymph node niches enhances type 1 immune responses to immunization |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2020-05-01 |
description |
Summary: Generating robust CD4+ T-helper cell type 1 (Th1) responses is essential for protective vaccine-induced type 1 immunity. Here, we examine whether immunization formulation associated with enhanced vaccine efficacy promotes antigen targeting and cell recruitment into lymph node (LN) niches associated with optimal type 1 responses. Immunization with antigen and Toll-like receptor agonist emulsified in oil leads to an increased differentiation of IFNγ/TNF-α+ polyfunctional Th1 cells compared to an identical immunization in saline. Oil immunization results in a rapid delivery and persistence of antigen in interfollicular regions (IFRs) of the LN, whereas without oil, antigen is distributed in the medullary region. Following oil immunization, CXCL10-producing inflammatory monocytes accumulate in the IFR, which mobilizes antigen-specific CD4+ T cells into this niche. In this microenvironment, CD4+ T cells are advantageously positioned to encounter arriving IL-12-producing inflammatory dendritic cells (DCs). These data suggest that formulations delivering antigen to the LN IFR create an inflammatory niche that can improve vaccine efficacy. |
topic |
vaccines emulsification lymph node interfollicular region chemokines CXCL10 |
url |
http://www.sciencedirect.com/science/article/pii/S221112472030632X |
work_keys_str_mv |
AT jeffreylian targetinglymphnodenichesenhancestype1immuneresponsestoimmunization AT aleksandrajozga targetinglymphnodenichesenhancestype1immuneresponsestoimmunization AT carolinelsokol targetinglymphnodenichesenhancestype1immuneresponsestoimmunization AT andrewdluster targetinglymphnodenichesenhancestype1immuneresponsestoimmunization |
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