Novel Approach of Differential Staining to Detect Necrotic Cells in Preimplantation Embryos
Background: This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst.Materials and Methods: Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidiu...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Royan Institute (ACECR), Tehran
2007-09-01
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Series: | International Journal of Fertility and Sterility |
Subjects: | |
Online Access: | http://www.ijfs.ir/article_45652_a2cceeef0127fc5afd520449b8ae1cd8.pdf |