Novel Approach of Differential Staining to Detect Necrotic Cells in Preimplantation Embryos

Background: This novel approach describes a rapid and simple method for identification of necrotic vs. viable cells within a mammalian blastocyst.Materials and Methods: Hatched bovine blastocysts produced in vitro were first incubated for 30 min in pre-equilibrated culture medium containing propidiu...

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Bibliographic Details
Main Authors: Seyyed Morteza Hosseini, Mehdi Hajian, Vajihe Asgari, Mohsen Forozanfar, Parvaneh Abedi, Mohammad Hossein Nasr-Esfahani
Format: Article
Language:English
Published: Royan Institute (ACECR), Tehran 2007-09-01
Series:International Journal of Fertility and Sterility
Subjects:
Online Access:http://www.ijfs.ir/article_45652_a2cceeef0127fc5afd520449b8ae1cd8.pdf