Identificazione rapida di mutazioni associate a farmaco-resistenza in ceppi di citomegalovirus umano mediante nPCR-RFLP

Identificazione rapida di mutazioni associate a farmaco-resistenza in ceppi di citomegalovirus umano mediante nPCR-RFLP

We developed a nested-PCR followed by restriction fragment length polymorphism (RFLP) for the detection of human cytomegalovirus (HCMV) UL97 M460V/I, H520Q, C592Q,A594V, L595S/F and C603W mutations associated to ganciclovir (GCV) resistance.The method uses five primer pairs and seven enzymes already...

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Bibliographic Details
Main Authors: Maria Cristina Medici, Monica Martinelli, Annalisa Aloisi, Laura Anna Abelli, Giuseppe Dettori, Carlo Chezzi
Format: Article
Language:English
Published: PAGEPress Publications 2007-03-01
Series:Microbiologia Medica
Subjects:
human cytomegalovirus (HCMV), nPCR-RFLP, ganciclovir resistance, transplant recipients
Online Access:http://www.pagepressjournals.org/index.php/mm/article/view/2892

Internet

http://www.pagepressjournals.org/index.php/mm/article/view/2892

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