Rapid Affinity Maturation of Novel Anti-PD-L1 Antibodies by a Fast Drop of the Antigen Concentration and FACS Selection of Yeast Libraries

The affinity engineering is a key step to increase the efficacy of therapeutic monoclonal antibodies and yeast surface display is the most widely used and powerful affinity maturation approach, achieving picomolar binding affinities. In this study, we provide an optimization of the yeast surface dis...

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Bibliographic Details
Main Authors: Biancamaria Cembrola, Valentino Ruzza, Fulvia Troise, Maria Luisa Esposito, Emanuele Sasso, Valeria Cafaro, Margherita Passariello, Feliciano Visconte, Maddalena Raia, Luigi Del Vecchio, Anna Morena D’Alise, Riccardo Cortese, Elisa Scarselli, Nicola Zambrano, Claudia De Lorenzo, Alfredo Nicosia
Format: Article
Published: Hindawi Limited 2019-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2019/6051870