RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells.
The innate immune system senses pathogens by pattern recognition receptors in different cell compartments. In the endosome, bacteria are generally recognized by TLRs; facultative intracellular bacteria such as Listeria, however, can escape the endosome. Once in the cytosol, they become accessible to...
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doaj-94dd9c09808641db9fd7e7e291ffe91c2020-11-25T01:00:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6287210.1371/journal.pone.0062872RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells.Cristina Amparo HagmannAnna Maria HerznerZeinab AbdullahThomas ZillingerChristopher JakobsChristine SchuberthChristoph CochPaul G HigginsHilmar WisplinghoffWinfried BarchetVeit HornungGunther HartmannMartin SchleeThe innate immune system senses pathogens by pattern recognition receptors in different cell compartments. In the endosome, bacteria are generally recognized by TLRs; facultative intracellular bacteria such as Listeria, however, can escape the endosome. Once in the cytosol, they become accessible to cytosolic pattern recognition receptors, which recognize components of the bacterial cell wall, metabolites or bacterial nucleic acids and initiate an immune response in the host cell. Current knowledge has been focused on the type I IFN response to Listeria DNA or Listeria-derived second messenger c-di-AMP via the signaling adaptor STING. Our study focused on the recognition of Listeria RNA in the cytosol. With the aid of a novel labeling technique, we have been able to visualize immediate cytosolic delivery of Listeria RNA upon infection. Infection with Listeria as well as transfection of bacterial RNA induced a type-I-IFN response in human monocytes, epithelial cells or hepatocytes. However, in contrast to monocytes, the type-I-IFN response of epithelial cells and hepatocytes was not triggered by bacterial DNA, indicating a STING-independent Listeria recognition pathway. RIG-I and MAVS knock-down resulted in abolishment of the IFN response in epithelial cells, but the IFN response in monocytic cells remained unaffected. By contrast, knockdown of STING in monocytic cells reduced cytosolic Listeria-mediated type-I-IFN induction. Our results show that detection of Listeria RNA by RIG-I represents a non-redundant cytosolic immunorecognition pathway in non-immune cells lacking a functional STING dependent signaling pathway.http://europepmc.org/articles/PMC3639904?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cristina Amparo Hagmann Anna Maria Herzner Zeinab Abdullah Thomas Zillinger Christopher Jakobs Christine Schuberth Christoph Coch Paul G Higgins Hilmar Wisplinghoff Winfried Barchet Veit Hornung Gunther Hartmann Martin Schlee |
spellingShingle |
Cristina Amparo Hagmann Anna Maria Herzner Zeinab Abdullah Thomas Zillinger Christopher Jakobs Christine Schuberth Christoph Coch Paul G Higgins Hilmar Wisplinghoff Winfried Barchet Veit Hornung Gunther Hartmann Martin Schlee RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells. PLoS ONE |
author_facet |
Cristina Amparo Hagmann Anna Maria Herzner Zeinab Abdullah Thomas Zillinger Christopher Jakobs Christine Schuberth Christoph Coch Paul G Higgins Hilmar Wisplinghoff Winfried Barchet Veit Hornung Gunther Hartmann Martin Schlee |
author_sort |
Cristina Amparo Hagmann |
title |
RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells. |
title_short |
RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells. |
title_full |
RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells. |
title_fullStr |
RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells. |
title_full_unstemmed |
RIG-I detects triphosphorylated RNA of Listeria monocytogenes during infection in non-immune cells. |
title_sort |
rig-i detects triphosphorylated rna of listeria monocytogenes during infection in non-immune cells. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
The innate immune system senses pathogens by pattern recognition receptors in different cell compartments. In the endosome, bacteria are generally recognized by TLRs; facultative intracellular bacteria such as Listeria, however, can escape the endosome. Once in the cytosol, they become accessible to cytosolic pattern recognition receptors, which recognize components of the bacterial cell wall, metabolites or bacterial nucleic acids and initiate an immune response in the host cell. Current knowledge has been focused on the type I IFN response to Listeria DNA or Listeria-derived second messenger c-di-AMP via the signaling adaptor STING. Our study focused on the recognition of Listeria RNA in the cytosol. With the aid of a novel labeling technique, we have been able to visualize immediate cytosolic delivery of Listeria RNA upon infection. Infection with Listeria as well as transfection of bacterial RNA induced a type-I-IFN response in human monocytes, epithelial cells or hepatocytes. However, in contrast to monocytes, the type-I-IFN response of epithelial cells and hepatocytes was not triggered by bacterial DNA, indicating a STING-independent Listeria recognition pathway. RIG-I and MAVS knock-down resulted in abolishment of the IFN response in epithelial cells, but the IFN response in monocytic cells remained unaffected. By contrast, knockdown of STING in monocytic cells reduced cytosolic Listeria-mediated type-I-IFN induction. Our results show that detection of Listeria RNA by RIG-I represents a non-redundant cytosolic immunorecognition pathway in non-immune cells lacking a functional STING dependent signaling pathway. |
url |
http://europepmc.org/articles/PMC3639904?pdf=render |
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