Frog oocytes to unveil the structure and supramolecular organization of human transport proteins.

Structural analyses of heterologously expressed mammalian membrane proteins remain a great challenge given that microgram to milligram amounts of correctly folded and highly purified proteins are required. Here, we present a novel method for the expression and affinity purification of recombinant ma...

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Bibliographic Details
Main Authors: Marc J Bergeron, Rajendra Boggavarapu, Marcel Meury, Zöhre Ucurum, Luc Caron, Paul Isenring, Matthias A Hediger, Dimitrios Fotiadis
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3131388?pdf=render