Protocol for efficient fluorescence 3′ end-labeling of native noncoding RNA domains

Protocol for efficient fluorescence 3′ end-labeling of native noncoding RNA domains

Noncoding RNAs (ncRNAs) comprise a class of versatile transcripts that are highly involved in the regulation of a wide range of biological processes. Functional long ncRNAs (> 200 nts in length) often adopt secondary structures that arise co-transcriptionally. To maintain the secondary structure...

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Bibliographic Details
Main Authors: Dahlia A. Awwad, A. Rachid Rahmouni, Fareed Aboul-ela
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:MethodsX
Subjects:
RNA
in vitro transcription
3′end-labeling
Fluorescence
Native purification
Chemical tagging
Online Access:http://www.sciencedirect.com/science/article/pii/S221501612030368X

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http://www.sciencedirect.com/science/article/pii/S221501612030368X

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