Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba)
In this work, the protein quality of different bean types after undergoing the preparatory methods of baking, cooking and extrusion was assayed. Protein quality was assessed using a rodent bioassay to evaluate growth and protein digestibility while amino acid composition was determined via HPLC. In...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2018-05-01
|
Series: | Nutrients |
Subjects: | |
Online Access: | http://www.mdpi.com/2072-6643/10/6/671 |
id |
doaj-950f872dd1f242c5a42a111426dbb050 |
---|---|
record_format |
Article |
spelling |
doaj-950f872dd1f242c5a42a111426dbb0502020-11-25T01:13:47ZengMDPI AGNutrients2072-66432018-05-0110667110.3390/nu10060671nu10060671Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba)Matthew G. Nosworthy0Gerardo Medina1Adam J. Franczyk2Jason Neufeld3Paulyn Appah4Alphonsus Utioh5Peter Frohlich6James D. House7Department of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaDepartment of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaFood Development Centre, Portage la Prairie, MB R1N 3J9, CanadaFood Development Centre, Portage la Prairie, MB R1N 3J9, CanadaCanadian International Grains Institute, Winnipeg, MB R3C 3G7, CanadaDepartment of Food and Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, CanadaIn this work, the protein quality of different bean types after undergoing the preparatory methods of baking, cooking and extrusion was assayed. Protein quality was assessed using a rodent bioassay to evaluate growth and protein digestibility while amino acid composition was determined via HPLC. In vivo protein digestibility was compared to an in vitro assessment method. The average protein digestibility corrected amino acid score (PDCAAS) for processed beans was higher than the digestible indispensable amino acid score (DIAAS) (61% vs. 45%). Extrusion/cooking of Phaseolus varieties resulted in higher PDCAAS (66% on average) and DIAAS values (61% on average) than baked (52% and 48%) while baked faba beans had higher PDCAAS (66%) and DIAAS (61%) values. A significant correlation was found between PDCAAS and in vitro PDCAAS (R2 = 0.7497). This demonstrates which bean processing method will generate the optimal protein quality, which has benefits for both industrial production and individual domestic preparation.http://www.mdpi.com/2072-6643/10/6/671bakingcookingextrusionin vitro protein digestibilitybeans |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Matthew G. Nosworthy Gerardo Medina Adam J. Franczyk Jason Neufeld Paulyn Appah Alphonsus Utioh Peter Frohlich James D. House |
spellingShingle |
Matthew G. Nosworthy Gerardo Medina Adam J. Franczyk Jason Neufeld Paulyn Appah Alphonsus Utioh Peter Frohlich James D. House Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba) Nutrients baking cooking extrusion in vitro protein digestibility beans |
author_facet |
Matthew G. Nosworthy Gerardo Medina Adam J. Franczyk Jason Neufeld Paulyn Appah Alphonsus Utioh Peter Frohlich James D. House |
author_sort |
Matthew G. Nosworthy |
title |
Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba) |
title_short |
Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba) |
title_full |
Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba) |
title_fullStr |
Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba) |
title_full_unstemmed |
Effect of Processing on the In Vitro and In Vivo Protein Quality of Beans (Phaseolus vulgaris and Vicia Faba) |
title_sort |
effect of processing on the in vitro and in vivo protein quality of beans (phaseolus vulgaris and vicia faba) |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2018-05-01 |
description |
In this work, the protein quality of different bean types after undergoing the preparatory methods of baking, cooking and extrusion was assayed. Protein quality was assessed using a rodent bioassay to evaluate growth and protein digestibility while amino acid composition was determined via HPLC. In vivo protein digestibility was compared to an in vitro assessment method. The average protein digestibility corrected amino acid score (PDCAAS) for processed beans was higher than the digestible indispensable amino acid score (DIAAS) (61% vs. 45%). Extrusion/cooking of Phaseolus varieties resulted in higher PDCAAS (66% on average) and DIAAS values (61% on average) than baked (52% and 48%) while baked faba beans had higher PDCAAS (66%) and DIAAS (61%) values. A significant correlation was found between PDCAAS and in vitro PDCAAS (R2 = 0.7497). This demonstrates which bean processing method will generate the optimal protein quality, which has benefits for both industrial production and individual domestic preparation. |
topic |
baking cooking extrusion in vitro protein digestibility beans |
url |
http://www.mdpi.com/2072-6643/10/6/671 |
work_keys_str_mv |
AT matthewgnosworthy effectofprocessingontheinvitroandinvivoproteinqualityofbeansphaseolusvulgarisandviciafaba AT gerardomedina effectofprocessingontheinvitroandinvivoproteinqualityofbeansphaseolusvulgarisandviciafaba AT adamjfranczyk effectofprocessingontheinvitroandinvivoproteinqualityofbeansphaseolusvulgarisandviciafaba AT jasonneufeld effectofprocessingontheinvitroandinvivoproteinqualityofbeansphaseolusvulgarisandviciafaba AT paulynappah effectofprocessingontheinvitroandinvivoproteinqualityofbeansphaseolusvulgarisandviciafaba AT alphonsusutioh effectofprocessingontheinvitroandinvivoproteinqualityofbeansphaseolusvulgarisandviciafaba AT peterfrohlich effectofprocessingontheinvitroandinvivoproteinqualityofbeansphaseolusvulgarisandviciafaba AT jamesdhouse effectofprocessingontheinvitroandinvivoproteinqualityofbeansphaseolusvulgarisandviciafaba |
_version_ |
1725160062856462336 |