Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2

Evaluation of a Lyophilized CRISPR-Cas12 Assay for a Sensitive, Specific, and Rapid Detection of SARS-CoV-2

We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the Lyo-CRISPR SARS-CoV-2 ki...

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Bibliographic Details
Main Authors: Lucía Ana Curti, Ivana Primost, Sofia Valla, Daiana Ibañez Alegre, Cecilia Olguin Perglione, Guillermo Daniel Repizo, Julia Lara, Ivana Parcerisa, Antonela Palacios, María Eugenia Llases, Adriana Rinflerch, Melanie Barrios, Federico Pereyra Bonnet, Carla Alejandra Gimenez, Débora Natalia Marcone
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Viruses
Subjects:
CRISPR
SARS-CoV-2
COVID-19
diagnosis
fluorescence detection
lysis buffer
Online Access:https://www.mdpi.com/1999-4915/13/3/420

Internet

https://www.mdpi.com/1999-4915/13/3/420

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