Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum

Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection by Leishmania (Leishmania) infantum

A kDNA PCR enzyme-linked immunosorbent assay (kDNA PCR-ELISA) for the diagnosis of human visceral leishmaniasis (HVL) was developed. The detection limit of the reaction, precision measurements, and cut-off of the kDNA PCR-ELISA were defined in a proof-of-concept phase. A reference strain of Leishman...

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Main Authors: Fernanda Alvarenga Cardoso Medeiros, Luciana Inácia Gomes, Edward Oliveira, Carolina Senra Alves de Souza, Maria Vitória Mourão, Gláucia Fernandes Cota, Letícia Helena dos Santos Marques, Mariângela Carneiro, Ana Rabello
Format: Article
Language:English
Published: Hindawi Limited 2017-01-01
Series:Journal of Tropical Medicine
Online Access:http://dx.doi.org/10.1155/2017/7364854

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http://dx.doi.org/10.1155/2017/7364854

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