A Novel and Efficient Method for Bacteria Genome Editing Employing both CRISPR/Cas9 and an Antibiotic Resistance Cassette

As Cas9-mediated cleavage requires both protospacer and protospacer adjacent motif (PAM) sequences, it is impossible to employ the CRISPR/Cas9 system to directly edit genomic sites without available PAM sequences nearby. Here, we optimized the CRISPR/Cas9 system and developed an innovative two-step...

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Bibliographic Details
Main Authors: Hong Zhang, Qiu-Xiang Cheng, Ai-Min Liu, Guo-Ping Zhao, Jin Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-05-01
Series:Frontiers in Microbiology
Subjects:
Online Access:http://journal.frontiersin.org/article/10.3389/fmicb.2017.00812/full