Fish oil protects against corn oil-induced cardiac insulin resistance and left ventricular dysfunction in rats via upregulation of PPAR-β/γ and inhibition of diacylglycerol/PCK axis activation

• Main Authors: Refaat A. Eid, Mubarak Al-Shraim, Samy M. Eleawa, Mohamed Samir Ahmed Zaki, Attalla Farag El-kott, Muhammad Alaa Eldeen, Mahmoud A. Alkhateeb, Mohammed Alassiri, Hussain Alderah
• Format: Article
• Language: English
• Published: Elsevier 2019-05-01
• Series: Journal of Functional Foods
• Subjects: Corn oil; Fish oil; Heart; Insulin; PPARs; Rats
• Online Access: http://www.sciencedirect.com/science/article/pii/S1756464619301458
• ISSN: 1756-4646

This study investigated the effects corn oil (CO) and different ratios of corn oil plus fish oil (FO) on insulin signaling in rat's heart. Adult male rats were fed low (LFD) or isocaloric high-fat diets (HFDs) of CO alone or CO + FO (ratios of 9:1 or 4:1). Cultured cardiomyocytes were treated with linoleic acid (LA), DHA + EPA (1:1) or LA + (DHA + EPA) (1:1). CO induced peripheral and cardiac insulin resistance with mitochondria damage, lipotoxicity activation of PKCε. It also upregulated LV levels of PPARα and down regulated PPARβ/δ. FO antagonized corn oil effects at both ratio with more profound effects with the ratio of 4:1. The regulatory roles of CO and FO on PPARs was shown in cultured cardiomyocytes and independent of hyperinsulinemia or free fatty acids. In conclusion, reducing the ratio of n-6/n-3 PUFAs in our diet toward a value of one is cardioprotective.