Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA
mRNA chimeras from chromosomal translocations often play a role as transforming oncogenes. However, cancer transcriptomes also contain mRNA chimeras that may play a role in tumor development, which arise as transcriptional or post-transcriptional events. To identify such chimeras, we developed a de...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2012-11-01
|
Series: | Neoplasia: An International Journal for Oncology Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558612801205 |
id |
doaj-95cc5d5c79234637a624d792b8851fe8 |
---|---|
record_format |
Article |
spelling |
doaj-95cc5d5c79234637a624d792b8851fe82020-11-25T00:59:00ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022012-11-0114111087109610.1593/neo.121342Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNARoberto Plebani0Gavin R Oliver1Marco Trerotola2Emanuela Guerra3Pamela Cantanelli4Luana Apicella5Andrew Emerson6Alessandro Albiero7Paul D Harkin8Richard D Kennedy9Saverio Alberti10Unit of Cancer Pathology, Centre of Excellence for Research on Aging, “G. D'Annunzio” University Foundation, Chieti, ItalyAlmac Diagnostics, Craigavon, United KingdomUnit of Cancer Pathology, Centre of Excellence for Research on Aging, “G. D'Annunzio” University Foundation, Chieti, ItalyUnit of Cancer Pathology, Centre of Excellence for Research on Aging, “G. D'Annunzio” University Foundation, Chieti, ItalyUnit of Cancer Pathology, Centre of Excellence for Research on Aging, “G. D'Annunzio” University Foundation, Chieti, ItalyUnit of Cancer Pathology, Centre of Excellence for Research on Aging, “G. D'Annunzio” University Foundation, Chieti, ItalyCINECA, Bologna, ItalyBMR Genomics, Padova, ItalyAlmac Diagnostics, Craigavon, United KingdomUnit of Cancer Pathology, Centre of Excellence for Research on Aging, “G. D'Annunzio” University Foundation, Chieti, ItalyUnit of Cancer Pathology, Centre of Excellence for Research on Aging, “G. D'Annunzio” University Foundation, Chieti, Italy mRNA chimeras from chromosomal translocations often play a role as transforming oncogenes. However, cancer transcriptomes also contain mRNA chimeras that may play a role in tumor development, which arise as transcriptional or post-transcriptional events. To identify such chimeras, we developed a deterministic screening strategy for long-range sequence analysis. High-throughput, long-read sequencing was then performed on cDNA libraries from major tumor histotypes and corresponding normal tissues. These analyses led to the identification of 378 chimeras, with an unexpectedly high frequency of expression (≈2 x 10-5 of all mRNA). Functional assays in breast and ovarian cancer cell lines showed that a large fraction of mRNA chimeras regulates cell replication. Strikingly, chimeras were shown to include both positive and negative regulators of cell growth, which functioned as such in a cell-type-specific manner. Replication-controlling chimeras were found to be expressed by most cancers from breast, ovary, colon, uterus, kidney, lung, and stomach, suggesting a widespread role in tumor development. http://www.sciencedirect.com/science/article/pii/S1476558612801205 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Roberto Plebani Gavin R Oliver Marco Trerotola Emanuela Guerra Pamela Cantanelli Luana Apicella Andrew Emerson Alessandro Albiero Paul D Harkin Richard D Kennedy Saverio Alberti |
spellingShingle |
Roberto Plebani Gavin R Oliver Marco Trerotola Emanuela Guerra Pamela Cantanelli Luana Apicella Andrew Emerson Alessandro Albiero Paul D Harkin Richard D Kennedy Saverio Alberti Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA Neoplasia: An International Journal for Oncology Research |
author_facet |
Roberto Plebani Gavin R Oliver Marco Trerotola Emanuela Guerra Pamela Cantanelli Luana Apicella Andrew Emerson Alessandro Albiero Paul D Harkin Richard D Kennedy Saverio Alberti |
author_sort |
Roberto Plebani |
title |
Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA |
title_short |
Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA |
title_full |
Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA |
title_fullStr |
Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA |
title_full_unstemmed |
Long-range Transcriptome Sequencing Reveals Cancer Cell Growth Regulatory Chimeric mRNA |
title_sort |
long-range transcriptome sequencing reveals cancer cell growth regulatory chimeric mrna |
publisher |
Elsevier |
series |
Neoplasia: An International Journal for Oncology Research |
issn |
1476-5586 1522-8002 |
publishDate |
2012-11-01 |
description |
mRNA chimeras from chromosomal translocations often play a role as transforming oncogenes. However, cancer transcriptomes also contain mRNA chimeras that may play a role in tumor development, which arise as transcriptional or post-transcriptional events. To identify such chimeras, we developed a deterministic screening strategy for long-range sequence analysis. High-throughput, long-read sequencing was then performed on cDNA libraries from major tumor histotypes and corresponding normal tissues. These analyses led to the identification of 378 chimeras, with an unexpectedly high frequency of expression (≈2 x 10-5 of all mRNA). Functional assays in breast and ovarian cancer cell lines showed that a large fraction of mRNA chimeras regulates cell replication. Strikingly, chimeras were shown to include both positive and negative regulators of cell growth, which functioned as such in a cell-type-specific manner. Replication-controlling chimeras were found to be expressed by most cancers from breast, ovary, colon, uterus, kidney, lung, and stomach, suggesting a widespread role in tumor development.
|
url |
http://www.sciencedirect.com/science/article/pii/S1476558612801205 |
work_keys_str_mv |
AT robertoplebani longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT gavinroliver longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT marcotrerotola longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT emanuelaguerra longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT pamelacantanelli longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT luanaapicella longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT andrewemerson longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT alessandroalbiero longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT pauldharkin longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT richarddkennedy longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna AT saverioalberti longrangetranscriptomesequencingrevealscancercellgrowthregulatorychimericmrna |
_version_ |
1725219276908920832 |