Emerging Methods for Efficient and Extensive Incorporation of Non-canonical Amino Acids Using Cell-Free Systems

Cell-free protein synthesis (CFPS) has emerged as a novel protein expression platform. Especially the incorporation of non-canonical amino acids (ncAAs) has led to the development of numerous flexible methods for efficient and extensive expression of artificial proteins. Approaches were developed to...

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Bibliographic Details
Main Authors: Yang Wu, Zhaoguan Wang, Xin Qiao, Jiaojiao Li, Xiangrong Shu, Hao Qi
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-07-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00863/full