The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availability

The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availability

By using a transcriptomic approach, we have elucidated the effect of Ni2+ on the global gene expression of S. pneumoniae D39 by identifying several differentially expressed genes/operons in the presence of a high extracellular concentration of Ni2+. The genes belonging to the AdcR regulon (adcRCBA,...

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Main Authors: Irfan eManzoor, Sulman eShafeeq, Muhammad eAfzal, Oscar P. Kuipers
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-12-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
Nickel
Zinc
pneumococcus
AdcR
Pht family proteins
AdcR regulon
Online Access:http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00091/full
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spelling doaj-95ef3fe0cef24ca5a09143135580898f2020-11-24T22:43:11ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882015-12-01510.3389/fcimb.2015.00091166613The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availabilityIrfan eManzoor0Irfan eManzoor1Sulman eShafeeq2Sulman eShafeeq3Muhammad eAfzal4Muhammad eAfzal5Oscar P. Kuipers6University of GroningenGovernment College University, FaisalabadUniversity of GroningenKarolinska InstitutetUniversity of GroningenGovernment College University, FaisalabadUniversity of GroningenBy using a transcriptomic approach, we have elucidated the effect of Ni2+ on the global gene expression of S. pneumoniae D39 by identifying several differentially expressed genes/operons in the presence of a high extracellular concentration of Ni2+. The genes belonging to the AdcR regulon (adcRCBA, adcAII-phtD, phtA, phtB, and phtE) and the PsaR regulon (pcpA, prtA, and psaBCA) were highly upregulated in the presence of Ni2+. We have further studied the role of Ni2+ in the regulation of the AdcR regulon by using ICP-MS analysis, electrophoretic mobility shift assays and transcriptional lacZ-reporter studies, and demonstrate that Ni2+ is directly involved in the derepression of the AdcR regulon via the Zn2+-dependent repressor AdcR, and has an opposite effect on the expression of the AdcR regulon compared to Zn2+.http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00091/fullNickelZincpneumococcusAdcRPht family proteinsAdcR regulon
collection DOAJ
language English
format Article
sources DOAJ
author Irfan eManzoor
Irfan eManzoor
Sulman eShafeeq
Sulman eShafeeq
Muhammad eAfzal
Muhammad eAfzal
Oscar P. Kuipers
spellingShingle Irfan eManzoor
Irfan eManzoor
Sulman eShafeeq
Sulman eShafeeq
Muhammad eAfzal
Muhammad eAfzal
Oscar P. Kuipers
The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availability
Frontiers in Cellular and Infection Microbiology
Nickel
Zinc
pneumococcus
AdcR
Pht family proteins
AdcR regulon
author_facet Irfan eManzoor
Irfan eManzoor
Sulman eShafeeq
Sulman eShafeeq
Muhammad eAfzal
Muhammad eAfzal
Oscar P. Kuipers
author_sort Irfan eManzoor
title The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availability
title_short The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availability
title_full The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availability
title_fullStr The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availability
title_full_unstemmed The regulation of the AdcR regulon in Streptococcus pneumoniae depends both on Zn2+- and Ni2+ -availability
title_sort regulation of the adcr regulon in streptococcus pneumoniae depends both on zn2+- and ni2+ -availability
publisher Frontiers Media S.A.
series Frontiers in Cellular and Infection Microbiology
issn 2235-2988
publishDate 2015-12-01
description By using a transcriptomic approach, we have elucidated the effect of Ni2+ on the global gene expression of S. pneumoniae D39 by identifying several differentially expressed genes/operons in the presence of a high extracellular concentration of Ni2+. The genes belonging to the AdcR regulon (adcRCBA, adcAII-phtD, phtA, phtB, and phtE) and the PsaR regulon (pcpA, prtA, and psaBCA) were highly upregulated in the presence of Ni2+. We have further studied the role of Ni2+ in the regulation of the AdcR regulon by using ICP-MS analysis, electrophoretic mobility shift assays and transcriptional lacZ-reporter studies, and demonstrate that Ni2+ is directly involved in the derepression of the AdcR regulon via the Zn2+-dependent repressor AdcR, and has an opposite effect on the expression of the AdcR regulon compared to Zn2+.
topic Nickel
Zinc
pneumococcus
AdcR
Pht family proteins
AdcR regulon
url http://journal.frontiersin.org/Journal/10.3389/fcimb.2015.00091/full
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