FACS-based isolation, propagation and characterization of mouse embryonic cardiomyocytes based on VCAM-1 surface marker expression.

Purification of cardiomyocytes from the embryonic mouse heart, embryonic stem (ES) or induced pluripotent stem cells (iPS) is a challenging task and will require specific isolation procedures. Lately the significance of surface markers for the isolation of cardiac cell populations with fluorescence...

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Bibliographic Details
Main Authors: Annica Pontén, Stuart Walsh, Daniela Malan, Xiaojie Xian, Susanne Schéele, Laura Tarnawski, Bernd K Fleischmann, Stefan Jovinge
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3875414?pdf=render